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Projekt B3: Von der makroskopischen bis zur molekularen Ebene: Innovative in-situ Methoden zur Untersuchung indirekter anthropogener Effekte in aquatischen Biofilmen
Zeitraum14. Nov. 2018 - 31. Dez. 2023
StatusAbgeschlossen
Der Einfluss indirekter anthropogener Effekte, insbesondere des Klimawandels sowie xenobiotischer Effekte durch Einbringung von Mikroplastik, auf Filmbildungsprozesse, Morphologie und Stabilität von aquatischen Biofilmen soll mittels innovativer in-situ Methoden untersucht werden. Beispielsweise führt Temperaturerhöhung zur Änderung der Biodiversität und Mikroplastik wirkt als Vektor für Schadstoffe und (ökosystemfremde) Mikroorganismen. Ziel der Untersuchungen ist die Unterscheidung von primären und sekundären Effekten sowie die Identifikation von Ursachen. Hierzu sollen dynamisch-mechanische Methoden, die integrale Informationen über die Stabilität und Haftung von Biofilmen liefern und Rasterkraftmikroskopie, welche Einblicke in die Haftung und Morphologie mit einer Ortsauflösung im sub-Mikrometer Bereich ermöglicht, durchgeführt werden. Mittels eines Fluoreszenzkorrelationsspektroskopie-Eigenbaus können durch selektive Markierung der Biofilmbestandteile Veränderungen der Wechselwirkungen der einzelnen Biosystemkomponenten mit Oberflächen und untereinander auf molekularer Ebene identifiziert werden.